Fig. 1
From: Non-invasive sources of cells with primary cilia from pediatric and adult patients
Cultures and images of URECs in 2D and 3D conditions. (A) Urine sample 24 h after collection at ×4 magnification. Asterisks indicate squamous cells, and black arrows indicate transitional cells. Scale bar 200 μm. Note that renal epithelial cells are not apparent. (B) Renal epithelial cells in culture 12 days after collection at ×4 magnification. Note that there are two morphologically distinct types of renal epithelial cells, marked with a white or black arrow. Scale bar 200 μm. (C) Wild-type UREC 3D spheroids. Megalin indicates that cells composing spheroids can be derived from the proximal tubule, while AQP2 was used as a marker for collecting duct cells. Nucleus (DAPI, blue); megalin and AQP2 (red); and ZO-1 (green). Scale bar 10 μm. (D) Wild-type UREC 3D spheroids. Cilia are indicated with white arrows. Nucleus (DAPI, blue); Ac. Tubulin (white); and ZO-1 (green). Scale bar 10 μm. (E) Wild-type URECs in 2D monolayer. Nucleus (DAPI, blue); Ac. Tubulin (white); pericentrin (PCNT, red). Scale bar 10 μm