Fig. 1

Rootletin expression pattern and knock-down efficiency. a Schematic of protein structure of Drosophila and human Rootletins. Regions of high probability of coiled-coil (grey) were found by MARCOIL (http://toolkit.tuebingen.mpg.de/marcoil). The ‘rootletin’ domain is shown in red. b–g Rootletin mRNA in embryos. b Wild type, stage 12. c Wild type, stage 14. d Wild type, stage 17, lch5 indicates lateral Ch organ clusters. e Higher magnification view of (d). e Rootletin knock-down, stage 17. g Higher magnification view of (f). h Wild-type stage 17 embryo of a Rootletin-lacZ enhancer reporter fusion, showing β-galactosidase immunoreactivity in Ch neurons (green), counterstained for sensory neurons with anti-Futsch antibody (magenta). Lch5, v’ch1 and vchAB are the designations of the abdominal Ch neurons. i Schematic representation of the Drosophila Ch organ structure with sensory cilia at the tips of the neuronal dendrites housed in a scolopale structure