Fig. 6

Transport of proteins in Rootletin knock-down Ch cilia. a, b Anti-HRP immunoreactivity (green) in Ch neurons of mature larvae, counterstained with anti-Futsch (magenta). a Wild type, two bands of anti-HRP staining are visible at the cilium base and surrounding a medial point in the cilium (white lines). Yellow line indicates approximate extent of the cilium (ci) at the tip of the dendrite inner segment (is). b The same bands are visible in Rootletin knock-down. c, d NompC localisation (green) in pupal Ch neuron cilia of Johnston’s organ; counterstained with anti-HRP (magenta). c Wild type. d Rootletin knock-down. e, f NompC localisation (green) in mature larval Ch neurons (lch5), counterstained with anti-HRP (magenta) (HRP bands indicated by white lines). e Wild type, the NompC is localised distally to the HRP immunoreactivity. f Rootletin knock-down, NompC is grossly normal, but shows some mislocalisation to the proximal cilium in one of the neurons. g, h RempA-YFP localisation (green) in mature larval Ch neurons (lch5), counterstained with anti-EYS (magenta) (HRP bands indicated by white lines). g Wild type, showing puncta of localisation at the ciliary dilation (white arrow). h Rootletin knock-down, showing no change in RempA-YFP localisation. All scale bars = 5 μm