Fig. 1From: Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in miceGenerating the Bbs10 −/− mouse model. a Schematic representation of the targeting strategy for Bbs10 allele. b Genotyping PCR results for homozygous knock out (Bbs10 −/−), heterozygous (Bbs10 +/−), wild-type (Bbs10 +/+) mice. PCR bands at 475 and 238 bp correspond to the excised allele and to the natural allele, respectively. (bp base pairs). c Relative mRNA expression levels of Bbs10 in kidneys and the eyes of Bbs10 +/+ and Bbs10 −/−. Reference gene Gapdh (n = 4 per genotype).Back to article page