Fig. 4

Renal phenotype of the Bbs10 ^−/− mice and Bbs10 ^fl/fl ; Cadh16-Cre ^+/− mice. a Pictures of hematoxylin and eosin-stained kidney sections of 3-month-old Bbs10 ^+/+ and Bbs10 ^−/− mice. b Transmission electron microscopy picture of kidney sections of the glomerular region from Bbs10 ^+/+and Bbs10 ^−/− mice (P podocyte, BM basement membrane). Scale bars 5 and 2 µm. c Microalbuminuria levels from Bbs10 ^+/+ and Bbs10 ^−/− mice. d Creatinine clearance levels (µL/min) and urinary volume under fluid deprivation of Bbs10 ^+/+ and Bbs10 ^−/− mice (n = 7–9). Values are expressed as mean ± SEM. e Transmission electron microscopy picture of kidney epithelial cells from Bbs10 ^+/+, Bbs10 ^−/− mice. Red arrows indicate the centrioles and the connecting cilium. f Transmission electron microscopy picture of kidney tubular cells from Bbs10 ^+/+, Bbs10 ^−/− mice. Red arrows indicate cytoplasmic vacuoles, A apical side, B Basolateral side. g Pictures of immunostained kidney sections for AQP2 (green) and β-tubulin (red) from Bbs10 ^+/+and Bbs10 ^−/− mice. Nuclei were counterstained with DAPI (blue). Scale bars 20 µm. Values are expressed as mean ± SEM. *p < 0.05