Fig. 4
From: Culture and detection of primary cilia in endothelial cell models
Use of co-labelling to identify primary cilia HUVEC and HMEC-1 primary cilia identification using both axonemal marker arl13b (a, b) and acetylated \(\alpha\)-tubulin marker 611b (a′, b′, white arrows). (a″, b″) Co-localisation of both antibodies indicates presence of cilium. a-a′) HUVECs grown in high serum (20 % FBS) confluent. b, b″ HMEC-1s are grown in low serum (2 % FBS) then serum-starved (0 % FBS for 48 h)