Fig. 5

Cilia incidence in HUVEC and HMEC-1. Low serum (2 % FBS) HUVECs did not express any cilia, whereas high serum (20 % FBS) HUVECs expressed cilia in all assessed conditions. High serum (20 % FBS) HUVECs had significantly lower cilia incidence than high serum (10 % FBS) HMEC-1s, in cobblestone population (p < 0.001). Cobblestone HMEC-1 cultures in low serum (2 % FBS) had significantly lower cilia incidence than high serum (10 % FBS) HMEC-1s (p < 0.001). Cilia incidence in high serum (20 % FBS) HUVECs is not significantly different in either confluent, serum-starved or cobblestone populations. Serum-starved and cobblestone low serum (2 % FBS) HMEC-1s have significantly higher cilia incidence than confluent low serum (2 % FBS) HMEC-1s (p < 0.001, for both). Cobblestone high serum HMEC-1s have significantly higher cilia incidence than confluent high serum HMEC-1s (p < 0.001). Boxplots the median with upper and lower quartiles, all data points have been plotted. Black dotted lines the predicted data point for that culture condition using the two-way interaction model. ***p < 0.001