Fig. 6From: Culture and detection of primary cilia in endothelial cell modelsCilia length of HUVEC and HMEC-1. Post-confluent conditions did not significantly affect HUVEC cilia length. Confluent high serum (20Â % FBS) HUVECs have longer cilia than confluent high serum HMEC-1s (10Â % FBS), (**p = 0.0032). Cilia length did not significantly differ between HMEC-1s grown in low (2Â % FBS) and high (10Â %) serum prior to confluence, regardless of post-confluent condition. In low serum HMEC-1s, cobblestone treatment resulted in longer cilia compared to confluent HMEC-1s (*p = 0.0309). In high serum HMEC-1s, both serum starvation (*p = 0.0267) nd cobblestone (*p = 0.0104) post-confluent treatments resulted in longer cilia compared to confluent HMEC-1s. Boxplot the median with upper and lower quartiles, all data points have been plotted. Data have a minimum of 13 cilia per condition, from a total of between 3 and 5 experimentsBack to article page