Fig. 2
From: Measuring the primary cilium length: improved method for unbiased high-throughput analysis
Visual appearance of the same cilium with the different methods. Visual appearance of the same angled cilium with the MIP method (a), PyT method (b) (see also Additional file 4: Movie S2), DAAS method (c), and a reconstruction with Amira (d). Scale bars are 3 μm. e. Length of a cilium, measured with different sizes of the z-slices using the PyT method. Dotted line indicates z-slice size of 0.25 µm, and dashed line indicates the cilium length (2.26 μm) measured by 3D reconstruction using Amira