Fig. 3
From: Measuring the primary cilium length: improved method for unbiased high-throughput analysis
The PyT and the DAAS methods. a. PyT method. With the assumption of a continuous slope, the Pythagorean theorem can be applied to calculate the length of a cilium. b–d. DAAS method. After selecting a cilium and the cross section line (b), the program gives a top view (c) and the vertical slice of the selected plane (d). Measurements are performed in Matlab. Cilia are measured on grayscales from 55 to 255