Fig. 1
From: Zebrafish: a vertebrate tool for studying basal body biogenesis, structure, and function
Reprinted from [15]. Reprinted from [16]
a–d Zebrafish transmission electron micrographs highlighting conserved BB structures: nine-triplet microtubule arrangement, TF transition fibers, DA distal appendages, DAV distal appendage vesicles. a Ultrastructure of the BBs and cilium from the zebrafish brain at 24 hpf. Scale bar 250 nm. b, c M-centrioles from zebrafish photoreceptors at 50 hpf. Scale bar 250 nm. d Schematic representation of zebrafish BB ultrastructure. e–g BBs and cilia can be simultaneously visualized in multiple zebrafish tissue types using GTU88 γ-Tubulin (BB) and acetylated α-Tubulin (cilia) antibodies. Fluorescent immunohistochemistry in the eye (e), pronephric duct (f), Kupffer’s vesicle (g) for BB (green), cilia (red) and nuclei (blue) in 24 hpf (e, f) 8 somite (g) embryos