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Fig. 2 | Cilia

Fig. 2

From: Using sea urchin gametes and zygotes to investigate centrosome duplication

Fig. 2

a Enucleated sea urchin zygote followed in vivo to characterize centrosome duplication. Each duplicated centrosome organizes a birefringent aster as visualized with a polarization microscope. The birefringence and appearance of the asters indicate that this enucleated zygote was in mitosis when the photograph was taken. The refractile sphere, slightly out of focus in the center of the cell, is a drop of the mineral oil used to cap the micropipette employed to enucleate the zygote. Scale divisions are 10 μm apart. b This particular zygote was recovered from the preparation, fixed, and serial semi-thick sectioned. Shown is a cross-section of a centriole in one of the centrosomes. The other centriole was found in a different section. Scale bar 0.1 μm

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