Fig. 5

Molecular characterization of the pocket. pH Smo cilia (green) were fixed and first labeled with anti-GFP (red) in unpermeabilized cells to label the extracellular region (arrowhead) and then fixed again, permeabilized and stained with various ciliary markers (blue). a Acetylated tubulin (Ac Tub) stains the entire length of the ciliary axoneme and does not correlate with anti-GFP staining. b The anti-GFP staining ends above the basal body (BB), marked by pericentrin. c The anti-GFP staining also ends above the transition zone (TZ), marked by CEP290. d–f The anti-GFP staining inversely coincides with: EHD1 (d), Septin 9 (e) and glutamylated tubulin (Glu Tub) (f)