Fig. 5
From: Fixation methods can differentially affect ciliary protein immunolabeling
Different fixation methods affect IFT88 immunolabeling at cilia in IMCD3 cells. IMCD3 cells were subjected to various fixation methods and then co-labeled with IFT88 (red) and acetylated-α-tubulin (green). All coverslips were also stained with Hoechst for nuclear labeling (blue). IFT88 labels the cilium in all fixation methods tested. Scale bars 5 µm