Table 1 Summary of fixation methods and their effects on cilia marker immunolabeling
From: Fixation methods can differentially affect ciliary protein immunolabeling
Antibodies | Cilia staining | ||||||
|---|---|---|---|---|---|---|---|
PFA-S | PFA/PBS | MeOH | CBPFA | CB MeOH | CB CBPFA | CB CBPFA MeOH | |
IMCD3 | |||||||
β-tubulin | + | + | + | + | ++ | +++ | +++ |
Acetylated-α- tubulin | +++ | +++ | ++ | +++ | +++ | +++ | +++ |
Detyrosinated tubulin | +++ | +++ | +++ | + | + | +++ | +++ |
Polyglutamylated tubulin | + | + | +/− | +++ | +++ | +++ | +++ |
AC3 | + | + | − | − | − | − | − |
Arl13b | +++ | +++ | + | +++ | − | − | − |
CSPP1 | +/− | +/− | – | ++ | – | + | +++ |
IFT20 | − | − | − | − | + | + | + |
RPE | |||||||
β-tubulin | +/− | + | + | + | ++ | +++ | +++ |
Acetylated-α-tubulin | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Detyrosinated tubulin | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Polyglutamylated tubulin | ++ | +++ | +/− | ++ | +++ | +/− | +++ |
AC3 | − | − | − | − | − | − | − |
Arl13b | +++ | +++ | − | +++ | − | − | − |
CSPP1 | + | + | − | +++ | − | +++ | + |
IFT20 | − | − | − | +/− | + | ++ | +++ |