Fig. 2

LARP6 is required for ciliogenesis in the neural tube. No cilia are detected in the neural tube of LARP6 morphants. conMO, L6MO and/or HA-hL6 mRNA were injected into two dorsal blastomeres of 4-cell stage embryos and fixed at stage 20. Cilia were stained by anti-acetylated α-tubulin antibody. Following staining, 100-μm transverse sections of specimens were prepared and images were taken by confocal microscopy. memRFP mRNA was injected as a lineage tracer. White dots indicate the lumen of the neural tube. The scale bar represents 10 μm. n indicates number of embryos examined