Fig. 4

LARP6 regulates the expression of mcidas in ciliogenesis. a The depletion of LARP6 blocked ciliogenesis in a Notch-independent manner. dn-mam1 RNA and/or L6MO with memRFP mRNA or conMO with memGFP mRNA were injected into the right side or the left side, respectively. Embryos were fixed at stage 25. Cilia were stained with an anti-acetylated tubulin antibody. Following staining, lateral explants were prepared from embryos and images were taken by confocal microscopy. The scale bar represents 20 μm. b Quantification of number of MCCs from a. Three embryos were randomly chosen from each of three independent experiments. *≤0.05, ****≤0.0001. c mcidas expression in LARP6 morphants at stage 14 by WISH. Images from the left (conMO) or right (L6MO) lateral side were taken from the same embryo. Red-gal signals indicate L6MO injected side. a anterior, p posterior, d dorsal, v ventral. d Rescue experiments with mcidas mRNA (500 pg/blastomere) in LARP6 morphant MCCs. e LARP6 does not control the expression of mab21-l3 and gmnc genes. L6MO was injected into two blastomeres of 2-cell stage embryos and RNAs were isolated at stage 13 for semi-quantitative RT-PCR. f The model of LARP6 function in ciliogenesis. LARP6 controls the expression of mcidas in a Notch-independent manner. n indicates number of embryos examined