Fig. 1

OFD1 depletion alters basal body positioning. a Projections of optical sections through the dorsal surface (cortex) and the cytoplasm of cells labeled with 1D5 (green), a marker of basal bodies and the anti-epiplasm antibody, a marker of the cortical units (red). OFD1 cell observed after one division upon OFD1 depletion. The depleted cell is smaller than the control. Numerous internal basal bodies are observed (empty arrowheads). b Magnification of a. The basal bodies (green) appear in the center of the units (red) in the wild-type cell. Basal bodies lying between units (arrows) and units devoid of basal bodies (filled arrowheads) are observed in the OFD1 depleted cell. c Optical transverse sections (top outside, bottom inside the cell) performed across the cell surface. In the control cell, the basal bodies are located at the center of the epiplasmic units (red). In the OFD1 depleted cell, basal bodies lying under the cell surface are observed (white arrows). Small epiplasmic units and epiplasmic units devoid of basal bodies are also observed (filled arrowhead)