Fig. 4

VFL3-A depletion alters basal body positioning. a Projections of confocal sections through the dorsal surface (cortex) and the cytoplasm of cells labeled with 1D5 (green), a marker of basal bodies and the anti-epiplasm antibody (red). VFL3-A cell observed after one division upon VFL3-A depletion. The depleted cell is smaller than the control. b Magnification of a. Singlets and some doublets appear in the center of the units in the wild-type cells. Numerous basal bodies doublets and some triplets (empty arrowheads) are observed in VFL3-A depleted cells. Some basal bodies are mispositioned (filled arrowhead). c Optical transverse sections (top outside, bottom inside the cell) performed across the cell surface. In the VFL3-A depleted cell, basal bodies lying under the cell surface are observed (arrow)