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Fig. 6 | Cilia

Fig. 6

From: Basal body positioning and anchoring in the multiciliated cell Paramecium tetraurelia: roles of OFD1 and VFL3

Fig. 6

The assembly of basal body appendages is impaired in VFL3-A depleted cells. Projection of confocal sections performed on a wild-type (WT) and VFL3-A depleted cells double labeled with KD2 (red), a marker of the striated rootlets and 1D5, a marker of basal bodies (green). WT a unique striated rootlet marks the basal bodies. All striated rootlets point to the anterior part of the cell (Ant). VFL3-A the basal body mispositioning is associated with the misalignment of the striated rootlets. Some basal bodies are completely devoid of striated rootlet (arrows) and other assemble several striated rootlets (arrowheads). b Double staining of WT and VFL3-A depleted cells by KD2 (red) and TEU318, a marker of basal bodies and microtubular rootlets. WT in addition to the striated rootlet, two microtubular rootlets mark the basal bodies (green). VFL3-A basal bodies devoid of striated rootlet are generally not associated with microtubular rootlets (arrows). Some basal bodies are associated with a single microtubular rootlet (white arrowhead). Pt posterior part of the cell

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