Fig. 7

Ultrastructural anomalies of basal bodies induced by VFL3-A depletion. a Cross section of wild-type cell (A1): one striated rootlet (SR) is associated with basal body, all pointing in the same direction (black arrows). VFL3-A the striated rootlets are disoriented (A2) and two or three rootlets can develop from a basal body (A3, A4). b Longitudinal sections of basal bodies in wild-type cell (B1) and VFL3 depleted cells observed after one division upon inactivation (B2, B3, B4). B1 Ultrastructure of non-ciliated and ciliated basal bodies in wild-type cell. B2 80% of the observed undocked basal bodies (n = 50) assemble normal pro-transition zone. B3, B4 These sections reveal fully mature new basal bodies that did not tilt up to a position parallel to their mother. c Transversal and longitudinal sections of basal bodies during the duplication process in wild-type cell (C1, C3) and VFL3-A depleted cells (C2, C4). Cells were observed during the first division upon inactivation. In the wild-type cell, duplicating basal body are aligned (C1, C3). In VFL3 depleted cell, the new basal bodies are not separated from the parental one. The most anterior one do not tilt up to a position parallel to the old one. An abnormal filamentous structure links new and ancient basal body (C2). The longitudinal section reveals a new basal body that have developed in abnormal orientation (C4). Ant anterior part, post-posterior part