Fig. 9

Localization of Myc-VFL3-1. Projections of confocal and corresponding STED sections performed on Myc-VFL3-1 expressing transformants stained by 1D5 (basal body) in red, anti-Myc (VFL3-1) in green and KD2 (striated rootlets) in white. The irregular KD labeling observed in confocal images appear as dots in STED imaging. The basal body microtubules visualized as single dots in confocal imaging appear as several puncta signals in STED imaging. VFL3-1 labeling localizes between the striated rootlet and the basal body microtubules to which they are attached. Right panel, scheme of the MYC-VFL3-1 localization at the basal body proximal region