Fig. 2

Absence of ARL13B labelling in adult tissue and single myocytes. Representative confocal images dual immunolabelled for ARL13B (red) and nuclei (blue). a Adult rat cardiac tissue and b adult rat cardiomyocyte. A scale bar is shown for each image. ARL13B labelling was completely absent from both adult rat cardiac tissue and cardiomyocytes. A high background autofluorescence at lower intensity shows tissue and myocyte structure, with no ARL13B antibody labelling