Fig. 10From: SF-Assemblin genes in Paramecium: phylogeny and phenotypes of RNAi silencing on the ciliary-striated rootlets and surface organizationThe presence of SR-type structures in the 30% Optiprep faction of cells expressing FLAG-SFA8a. The cells homogenating from FLAG-SFA8a-expressing cells prepared as described in “Materials and methods” are separated by density on an Optiprep step gradient. a No immunofluorescent structure in the 20% Optiprep fraction embedded in agarose. b A representative immunofluorescence image of what appears to be a FLAG-SFA8a-labeled SR from the 30% Optiprep fraction embedded in agarose. Scale bar is 2 μmBack to article page