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Fig. 10 | Cilia

Fig. 10

From: SF-Assemblin genes in Paramecium: phylogeny and phenotypes of RNAi silencing on the ciliary-striated rootlets and surface organization

Fig. 10

The presence of SR-type structures in the 30% Optiprep faction of cells expressing FLAG-SFA8a. The cells homogenating from FLAG-SFA8a-expressing cells prepared as described in “Materials and methods” are separated by density on an Optiprep step gradient. a No immunofluorescent structure in the 20% Optiprep fraction embedded in agarose. b A representative immunofluorescence image of what appears to be a FLAG-SFA8a-labeled SR from the 30% Optiprep fraction embedded in agarose. Scale bar is 2 μm

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