Fig. 4

Structural Group depletion that can lead to basal body row misalignment and abnormal SR appearance. a, c Control and Structural Group 2 (also Paralog Group 7)-depleted cells, respectively. The yellow box in each image is enlarged (b, d) to highlight basal body rows. The basal bodies are green (ID5 antibody) and SRs are red (anti-SR). a, b Straight rows of basal bodies as well as SR rows that extend between the posterior pole and the anterior pole. c, d The severely misaligned basal body rows as well as abnormal SRs (shorter and not pointed toward the anterior pole) and disrupted SR rows. All images are of the dorsal surfaces, but misalignments can occur anywhere on the surface except the oral groove. Scale bars are 15 μm (a, c) and 3 μm (b, d)