Fig. 7

Immunofluorescence images demonstrating that the cells depleted of Structural Groups are ciliated and darkfield images show effects of depletion on swimming behavior. In a and b, immunofluorescence images of Control cells show straight basal body rows (red) and cilia (green), respectively. In b and d immunofluorescence images of Structural Group 1-depleted cells show that the cells have cilia (green) and misaligned basal body rows (red). In a and c, areas pointed out by white arrows are blown up in e and f showing that cilia emanate from basal bodies of both Control and Structural Group 1-depleted cells, even in an area with misaligned basal bodies. Scale bars are 15 μm (a–d) and 3 μm (e, f). g, h The swimming patterns of cells taken by darkfield microscopy. We show here examples of Control and Structural Group 1-depleted cells. Scale bar is 1 mm