Fig. 8

Depletion of SFA proteins leaving the angles between rootlets unaffected. Images show the staining of all three rootlets [TR (green), PR (green) and SR (red)] along with basal bodies (green) in the control, Paralog Group 2-depleted cell, Structural Group 1-depleted cell or Structural Group 2-depleted cell. Scale bars are 3 μm. White circles denote the position of the basal bodies with all three rootlets visible that were used in measuring the angles between rootlets. It took more images to reach 100 basal body measurements from the misaligned areas because we did not want to skew the measurements in these areas with basal bodies from normal rows