Figure 2

Centrosomes at the spindle poles of the blastomere of an eight- or thirty-two-cell paraformalin-fixed zebrafish embryo depicted by γ-tubulin antibody GTU-88 or TU-30, respectively, and Alexa Fluor 488 dye-conjugated second antibody (green) as well as chromosomes stained with 4',6-diamidino-2-phenylindole (blue). Z-axis projections of approximately 60 optical sections photographed with an Olympus spinning disk confocal microscope using a 60 × oil immersion lens objective. Note the size differences, with the γ-tubulin array being smaller in centrosomes of 32-cell compared to 8-cell embryos.