Figure 2
From: The primary cilium functions as a mechanical and calcium signaling nexus
PC2, TRPV4, and PIEZO1 localize to MLO-Y4 primary cilia and plasma membrane. (A – C) Localization of PC2, TRPV4, and PIEZO1 on MLO-Y4 primary cilia and plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, and actin protein in MLO-Y4s treated with scrambled control siRNA and Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), and Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM.