Fig. 5
From: Current topics of functional links between primary cilia and cell cycle
Effects of trichoplein depletion on cell cycle regulators. Proliferating RPE1 cells were transfected with siRNA for control or trichoplein (#1 and #2), and then cultured for 72Â h. The cell extracts were subjected to immunoblotting analysis with p27Kip1, p53, p21Cip1, p16INK4a, trichoplein, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). As controls of immunoblotting with p27Kip1 or p53/p21Cip1, RPE1 cells were subjected to 72Â h serum starvation or UV irradiation (10Â J/m2, 24Â h), respectively. These are original data for this review