Figure 9
From: Development of a method for the measurement of primary cilia length in 3D
2D maximum projection (MP) and 3D visualization of primary cilia. (A, B) In vitro chondrocyte primary cilia (arrow) labeled with acetylated α-tubulin. (A) 2D maximum projection image, (B) the corresponding 3D projection and skeletal representation. (C, D) In situ kidney primary cilia viewed as 2D and 3D images. (C, D; arrows) two intertwined cilia that cannot be distinguished in 2D or 3D; (C, D; arrowhead) two cilia that overlap in the 2D MP image that can clearly distinguished in the 3D view. Scale bars = 5 μm.