ciliaFA: a research tool for automated, high-throughput measurement of ciliary beat frequency using freely available software

Cilia

Table 1 The ependymal ciliary beat frequency (CBF) determined by two observers using high-speed digital imaging and the ciliaFA software

Time	CBF (Hz)
	ciliaFA	Direct counting, observer 1	Direct counting, observer 2	Observer 1 (second count)
0 minutes	43.05	39.06	42.37	47.17
	42.55	37.87	41.67	40.32
	45.25	43.85	44.64	44.64
	42.40	39.68	41.67	39.68
	43.07	41.66	42.37	41.67
	43.31	38.46	40.98	46.29
	39.80	33.78	37.88	39.68
	40.30	30.86	32.00	30.86
5 minutes	18.86	18.79	18.79	18.25
	24.28	23.36	23.15	23.15
	20.78	21.32	22.52	24.75
	18.97	18.79	18.66	18.66
	18.71	18.79	18.51	18.94
	18.82	18.79	18.66	18.51
	20.77	22.12	23.15	22.52
	30.43	32.00	30.86	30.86
ciliaFA-observer variability		1.42 Hz	0.84 Hz	-
Interobserver variability			1.17 Hz	-
Intraobserver variability				1.08 Hz

The effect of the addition of the bacterial toxin pneumolysin was monitored over time. Data are from eight regions of interest (ROI) at each time point.

ISSN: 2046-2530