Cluap1 localizes to primary cilia in vitro. Antibody against acetylated α-tubulin (red) and Cluap1 (green) label primary cilia (arrows) in (A-C) NIH3T3 cells (scale bars are 14 μm). (D-F) 176-6C collecting duct epithelium (scale bars are 21 μm) and (G-I) IMCD3 cells (scale bars are 20 μm). Arrows indicate primary cilium. Nuclei are stained blue with Hoechst.