Figure 5From: Mammalian Clusterin associated protein 1 is an evolutionarily conserved protein required for ciliogenesisCluap1KOembryos have defects in floorplate induction. (A,C,E) Cluap1WT E9.5 embryos stained for Arl13b (green) show cilia in the neural tube and surrounding tissue. Staining for Sonic hedgehog ligand (red) shows a Shh immunopositive floorplate. (B,D,F) Cluap1KO embryos show an absence of cilia as indicated by the lack of Arl13b staining. Note the lack of a clearly defined Shh immunopositive floorplate. Hoechst nuclear stain in blue. Scale bars are 21 μm.Back to article page