Somatostatin 3 receptor fused to green fluorescent protein labels cilia in IMCD3 cells. (A) Confocal image of IMCD3 cell expressing SsTR3-GFP in cilia. (B-I) Live imaging of a dividing IMCD3 cell with SsTR3-GFP expressed in cilia over 15 hours. Red dashes outline individual cells. Of the two daughter cells, one expresses GFP in cilia ahead of the other daughter cell (F-I; yellow arrow). The associated movie was imaged at a rate of one frame every 10 min. (J) Number of IMCD3 cells expressing SsTR3-GFP in cilia during live imaging observation. Of the divisions, 93% resulted in one daughter cell expressing SsTR3-GFP prior to the other daughter cell. Bar is 10 μm (A and B-I).