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Characterising a novel mouse model with a mutated ciliopathy gene (Cep290) leading to Joubert Syndrome

Joubert syndrome (JBTS) is an inherited ciliopathy leading to a cerebellum-retinal-renal syndrome. Recent genetic advances have allowed positional cloning and identification of numerous JBTS genes. CEP290, one of the JBTS genes identified, (alias NPHP6) encodes a centrosomal protein and accounts for 7% of patients with Joubert syndrome. We have identified a murine Embryonic Stem (ES) cell line containing a Cep290 “gene trap” using data base searches. ES cells were cultured before injecting into murine blastocysts to create chimaeric mice. Chimeras were bred to produce viable, healthy heterozygous mutant mice. Heterozygous mutant mice have been intercrossed to produce mice homozygous for the Cep290 truncating mutation. Cep290-/- animals (homozygous for the gene trap Cep290) exhibit a cortico-medullary cystic kidney disease commencing from birth. Histological examination reveals that these cysts are collecting duct in origin, staining positively for aquaporin-2 and -3. In this study the cilia were investigated in cystic Cep290-/- animals using Electron Microscopy (EM) analysis. Scanning electron microscopy (SEM) identified that cilia were evident within renal tubules in Cep290-/- animals. Once cilia were identified in Cep290-/- animals Transmission Electron Microscopy (TEM) was carried out to investigate cross sections of the collecting duct cilium in cystic and non-cystic kidneys. TEM analysis identified tubular basement membrane disruptions in Cep290-/- animals. The Cep290-/- mouse described provides an excellent model to investigate the mechanisms involved in cyst formation and to test novel therapeutic agents.

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Correspondence to AM Hynes.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hynes, A., Miller, C., Eley, L. et al. Characterising a novel mouse model with a mutated ciliopathy gene (Cep290) leading to Joubert Syndrome. Cilia 1 (Suppl 1), P89 (2012).

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  • Embryonic Stem
  • Embryonic Stem Cell
  • Transmission Electron Microscopy Analysis
  • Truncate Mutation
  • Gene Trap