Localization of DHC1b in wild-type, fla24 , and fla24 revertants at 21°C and 32°C. DHC1b staining is shown in green (first column) and the flagella are labeled with acetylated α-tubulin (red, second column). Merged images of both staining are shown in the third column. Overexposed DHC1b signals are shown in the forth column to show the localization of DHC1b in the flagella. Cells were obtained from 21°C and various time points at 32°C, as indicated. (A) Wild-type and fla24 cells. (B) fla24 revertants. (C) Twenty micrograms of flagellar proteins were isolated from wild-type (FLA24) and fla24 cells at 21°C, and probed with DHC1b and D1bLIC antibodies. The membrane that used to probe D1bLIC was stripped and reprobed with α-tubulin to normalize the loading.