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Table 4 Reversion of fla18 allele provides evidence of causality

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Strain name MboII Sequence for digestion (underline)a Amino acid Sanger sequence
Wild-type Cut GA A GAAGGCAGAT Glutamic acid GAG
fla18 Not cut GAGGAAGGCAGAT Glycine GGG
R1 Cut GA A GAAGGCAGAT G to E GGG to GAG
R2 Cut GA A GAAGGCAGAT G to E GGG to GAG
R3 Cut GA A GAAGGCAGAT G to E GGG to GAG
R5 Cut GA A GAAGGCAGAT G to E GGG to GAG
R6 Cut GA A GAAGGCAGAT G to E GGG to GAG
R7 Cut GA A GAAGGCAGAT G to E GGG to GAG
R8 Cut GA A GAAGGCAGAT G to E GGG to GAG
R9 Cut GA A GAAGGCAGAT G to E GGG to GAG
  1. aUnderlined nucleotides in the third column indicate the recognition site. This site is created by the primers in the wild-type (FLA18) sequence but not in the mutant (fla18) sequence.