Table 4 Reversion of fla18 allele provides evidence of causality
From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas
Strain name | MboII | Sequence for digestion (underline)a | Amino acid | Sanger sequence |
|---|---|---|---|---|
Wild-type | Cut | GA A GAAGGCAGAT | Glutamic acid | GAG |
fla18 | Not cut | GAGGAAGGCAGAT | Glycine | GGG |
R1 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R2 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R3 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R5 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R6 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R7 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R8 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |
R9 | Cut | GA A GAAGGCAGAT | G to E | GGG to GAG |