Figure 10

IFT172 is part of the fibrous core of pf19 flagella. (A) Confocal (a, c, e, g) and STED (b, d, f, h) images of WT flagella stained with anti-β-tubulin (a, c, e, g) and anti-IFT172 (b, d, f, h). Cells were extracted and fixed either sequentially (a-d) or simultaneously (e-h). Arrowheads in h: IFT172-containing particles flanking a central rod containing IFT172; note the absence of such particles in the flagella (b and d) derived from cells extracted first with detergent. Note also that the central IFT172-containing core, present in pf19 flagella (d) but not WT flagella (b), persists after detergent extraction (h) and is absent from the distal end of the cilium (compare c and d). (B) Overview of the cell corresponding to g/h showing the IFT172 signal in STED (a) and confocal illumination (b) and the tubulin signal in confocal illumination (c). Arrowheads: IFT172-containing particles flanking the central rods (arrows), which are stained strongly by the IFT172 antibody. (C) WT and pf19 gametes (a) and the resulting zygotes (b–e) were stained with antibodies to hydin (green) and IFT172 (red); DAPI staining is shown in blue. Open arrowheads: flagella of pf19 gametes (a) or zygotic flagella derived from pf19 (b–d). Closed arrowheads: flagella of WT gametes (a) or zygotic flagella derived from WT (b–d). Small arrows in d: residual IFT172 flanking the developing CP as visualized by anti-hydin. Bar = 5 μm.