Figure 5
From: Flagellar central pair assembly in Chlamydomonas reinhardtii
Localization of hydin in pf18 x wild type dikaryons. Zygotes from a mating of pf18 with a WT strain expressing α-tubulin fused to a triple HA-tag were analyzed by immunofluorescence microscopy using anti-α-tubulin (a1, b1), anti-HA (a2, b2), and anti-hydin (a3, b3) or anti-HA (c1) and anti-PF6 (c2). Merged images are shown in a4, b4, and c3. Arrows: flagella derived from pf18. Open arrowheads: incorporation of HA-tubulin at the distal end of flagella derived from pf18. Filled arrowheads: hydin (a3, b3) or PF6 (c2) in flagella derived from pf18. CP assembly is not apparent in flagella derived from pf18. Bar = 5 μm.