Figure 3
From: Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium
HIF-2α accumulates in the cilium with IL-1β treatment. (A) Whole cell HIFα expression (green), shown by immunofluorescent labelling, co-localised to nucleus (blue) in bovine chondrocytes. (B) Field images (large left-hand panels) and single cilium images (three small panels) showing varying degrees of HIF-2α expression in the cilium: negative cilia localisation (top), basal only localisation in samples treated with IL-1β (middle) and axonomal and basal staining with exposure to IL-1β (bottom). Single cilia images correspond to white boxes in field images. (C) Percentage of cilia exhibiting either basal only or axonomal and basal localisation of HIF-2α in untreated control cells or cells treated for 6 h with IL-1β (10 ng.mL-1), n shown above bars, fisher’s exact test statistics in text. (D) HIF-2α co-localised to primary cilium in human articular chondrocyte treated with IL-1β for 24 h.