Figure 4

Enhanced cytokine levels in response to S. pneumoniae infection. Levels of IL-6 (A), TNF (B), IL-2 (C), IFNγ (D), IL-4 (E), IL-17 (F), and IL-10 (G) were measured in wild type, nm1054, and bgh lung homogenates by flow cytometry using a cytometric bead array mouse Th1/Th2/Th17 cytokine kit. Mice were infected with S. pneumoniae on day 0, and cytokine levels were measured on days 0, 1, 2, and 3. Cytokine levels are normalized to internal standards. Statistical significance relative to wild type (P <0.05) is indicated by an asterisk.