Figure 2

MKS3- depleted cells have sparse cilia and distorted cell andciliary surfaces. Scanning electron micrographs of representative imagesof control (A) and (B), MKS3-depleted(C) through (F) and IFT88-depleted(G) and (H) cell populations. The cell in(G) has been rotated. Whole-cell images of control (A),MKS3-depleted (C) and (E) andIFT88-depleted (G) cells. Scale bars: 10 μm.The control cell (A) is covered in cilia, whereas theMKS3-depleted cells show sparse and short cilia(C) and (E). The IFT88-depleted cell(G) has almost no cilia remaining, although a few short cilia arevisible (yellow arrows). Higher magnifications of the cell surfaces from(A), (C), (E) and (G) (yellow boxes)are shown in (B), (D), (F) and (H), respectively(scale bars: 2 μm). Control cell surface (B) shows ciliaarising from the cortical units, whereas the MKS3-depleted cells showshort and missing cilia (D) and (F). Over 50% of theMKS3-depleted cells have cilia that are severely misshapen and“blebby.” The cell surfaces of these cells also became heavilywrinkled and distorted (F). The IFT88-depleted cell(H) shows normal organization of cortical units.