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PI3K-C2α regulates Polycystin-2 ciliary entry to prevent kidney cyst formation
Cilia volume 4, Article number: O1 (2015)
PI3K-C2α is a regulator of vesicle recycling at the base of the primary cilium and is required for the targeting of ciliary components. Here we sought to understand whether PI3K-C2α is required for the targeting of Polycystin-2 to primary cilia and the consequent regulation of kidney cyst formation.
Homozygous mutation of Pik3c2a, the gene encoding for PI3K-C2α, is embryonic lethal. Hence, the function of PI3K-C2α in kidney cilia has been studied both in vitro, in Pik3c2a-silenced IMCD3 cells, and in vivo, in Pik3c2a-heterozygous mice, using an Ischemia/Reperfusion model of renal injury.
PI3K-C2α resides at the recycling endosome compartment surrounding the primary cilium base where it controls the activation of Rab8, a key mediator of cargo protein targeting to the primary cilium. Consistently, partial reduction of PI3K-C2α is sufficient to impair elongation of the cilium both in Pik3c2a-silenced IMCD3 cells and in kidney tubules of Pik3c2a+/- mice. Importantly, absence of PI3K-C2α impairs the Rab8-dependent transport of Polycystin-2 to cilia and produces an overactivation of proliferative pathways regulated by ciliary Polycystins, such as the mTOR and MAPK pathways. Both defects can be rescued by transfection of constitutively active Rab8. In line with defective Polycystin signaling, heterozygous deletion of PI3K-C2α in mice causes an overall deregulation of proliferative signals in response to Ischemia/Reperfusion-induced renal damage, and this condition predisposes to cyst development.
These results indicate that PI3K-C2α is required for the transport of ciliary components like Polycystin-2 and that reduction in PI3K-C2α levels is sufficient to enhance susceptibility to cystic kidney disease.
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Margaria, J., Franco, I., Ranghino, A. et al. PI3K-C2α regulates Polycystin-2 ciliary entry to prevent kidney cyst formation. Cilia 4, O1 (2015). https://doi.org/10.1186/2046-2530-4-S1-O1
- MAPK Pathway
- Renal Injury
- Primary Cilium
- Homozygous Mutation
- Proliferative Signal