Volume 4 Supplement 1

Proceedings of the Second International Cilia in Development and Disease Scientific Conference (2014)

Open Access

Ultrastructural studies of Intraflagellar Transport trains in Chlamydomonas reinhardtii suggest a revision of the current model for IFT trafficking in the flagellar compartment

  • E Vannuccini1,
  • E Paccagnini1,
  • F Cantele2,
  • M Gentile1,
  • D Dini1,
  • F Fino1,
  • C Mencarelli1 and
  • Pietro Lupetti1
Cilia20154(Suppl 1):O16

https://doi.org/10.1186/2046-2530-4-S1-O16

Published: 13 July 2015

Intraflagellar Transport (IFT) is the molecular process responsible for the active bidirectional trafficking of structural and functional components that occurs in the flagellar compartment of eukaryotic cells. Flagellar components undergo a constant turnover at flagellar tip and multiple evidences indicate that flagellar elongation, maintenance and reabsorption depend on the correct balance between anterograde and retrograde trafficking. IFT particles are formed by >22 polypeptides assembled into two subcomplexes, A and B, and are moved bidirectionally along the outer surface of axonemal doublets as linear rows of IFT particles, for which we proposed the term "train". Anterograde IFT trains are moved by kinesin II and carry to flagellar tip the retrograde motor cytoplasmic dynein 1b, responsible for retrograde IFT. In a previous study carried out on Chlamydomonas flagella we identified two types of IFT trains we named long and short trains, each characterized by a specific ultrastructure and a definite internal repeat, and proposed that long, less compact trains could represent anterograde IFT while the short, more compact trains could be retrograde. To challenge such model, we monitored by transmission electron microscopy the IFT trains expressed both in wt regenerating flagella and during flagellar reabsorption induced in the ts mutant pf1-fla10. We also progressed in our electron tomographic 3D modeling of short IFT trains. Our data suggest that long IFT trains are not the only anterograde IFT component. Rather, anterograde IFT is contributed also by a subclass of short trains that is expressed in a flagellar length-dependent fashion.

Authors’ Affiliations

(1)
Dept. of Life Sciences, University of Siena
(2)
Dipartimento di Chimica Università di Milano

Copyright

© Vannuccini et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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