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  • Open Access

A systems biology approach towards the prediction of ciliopathy mechanisms

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Cilia20154 (Suppl 1) :P88

  • Published:


  • System Biology
  • Genetic Data
  • Cellular Mechanism
  • Sequencing Technology
  • Clinical Phenotype


Understanding the molecular and cellular mechanisms of ciliopathies is vital for dissecting their pathogenesis, identifying appropriate therapeutic targets and designing effective treatments. Recent advances in DNA sequencing technology have provided a torrent of genetic data that can now be used to elucidate the genetic basis of human diseases. A consensus has emerged among biologists that to fully exploit the available data, they have to be correlated with additional research. This is especially important for rare-disease genetics, because of the small number of available patients.


Computational methods could be employed to tackle this problem. Integration of diverse biological data can provide additional evidence to support experimental observations. We present a statistical method that predicts disease causing genes by integrating protein interaction data and clinical phenotype annotations. For a given protein in the network the method predicts the clinical phenotypes that may appear upon protein alteration based on the phenotypes associated with the neighbours of the protein.


Application to protein interaction data from the SYSCILIA project ( and phenotype annotations from the HPO project ( succesfully predicts candidate Nephronophthisis genes. The results also imply that abnormal Hh signaling may be the cause of Nephronophthisis and GPCR mislocalization a possible way by which cilia defects affect Hh signaling.


The results indicate that the developed method can be useful for the dissection of disease pathogenesis by predicting disease genes and drawing new hypotheses on the underlying mechanisms. Such hypotheses can assist in the design of new targeted experiments.

Authors’ Affiliations

Institute of Systems and Synthetic Biology, Genopole, CNRS, University of Evry, Evry, France
Centre for Molecular and Biomolecular Informatics, Radboud University Nijmegen, Nijmegen, The Netherlands
European Molecular Biology Laboratory, University of Heidelberg, Heidelberg, Germany
Cell Networks, Bioquant, Cluster of Excellence, University of Heidelberg, Heidelberg, Germany
Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
Department of Human Genetics, Radboud University Medical Center, Radboud, The Netherlands
Division of Experimental Ophthalmology and Medical Proteome Center, Eberhard-Karls Universität Tübingen, Tübingen, Germany
Syscilia, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands


© Koutroumpas et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.