Figure 5
Phylogenetic analysis of Ca 2+ -binding proteins. Proteins were aligned by CLUSTALW, and the tree was constructed by MEGA5. Ciona parvalbumin-like protein (XP_002129217) was used as the outgroup. The value shown on each branch represents the number of times that a node was supported in 1,000 bootstrap pseudo-replications. Sequences were obtained from the organisms Ciona (Ciona intestinalis), human (Homo sapiens), fungus (Batrachochytrium dendrobatidis), Naegleria (Naegleria gruberi), Euglena (Euglena gracilis), Trypanosoma (Trypanosoma cruzi or T. brucei), Giardia (Giardia intestinalis or G. lamblia), Trichomonas (Trichomonas vaginalis), Chlamydomonas (Chlamydomonas reinhardtii), Paramecium (Paramecium tetraurelia), and Ectocarpus (Ectocarpus siliculosus). The sources of amino acid sequences are as follows: Ciona calmodulin (AB076905), Ciona calaxin (AB079059), Ciona centrin (XP_004227465), Ciona NCS-1 (XP_002126443), Ciona CNB (XP_002130765); human CaM (CAA36839), human calaxin (NP_078869), human NCS1 (NP_055101), human CNB (NP_000936), human centrin (NP_004057); chytrid fungus calaxin (XP_006677085), chytrid fungus CaM (XP_006678916), chytrid fungus centrin (XP_006682970), chytrid fungus NCS1 (XP_006675998), chytrid fungus CNB (XP_006677028); Naegleria CaM (XP_002683533), Naegleria centrin (XP_002678269); Trypanosoma CaM (XP_805243), Trypanosoma centrin (XP_805423), Trypanosoma calflagin (Q26680); Euglena CaM (P11118), Euglena centrin (AGS09408); Giardia CaM (XP_001705820), Giardia centrin (XP_001707577), Giardia LC4 (XP_001705117); Trichomonas CaM (XP_001326924), Trichomonas centrin (CAB55607), Trichomonas CNB (XP_002680632); Paramecium CaM (XP_001448363), Paramecium LC4 (XP_001442002), Paramecium centrin (XP_001347281), Paramecium DC3 (XP_001444482); Ectocarpus LC4 (CBN80105), Ectocarpus CaM (CBN74265), Ectocarpus centrin (CBN79657), Ectocarpus DC3 (CBJ30770). The protein sequences with specific accession numbers were obtained from DDBJ/EMBL/GenBank, or from genome browsers with the following URLs: Chlamydomonas http://genome.jgi-psf.org/Chlre4/Chlre4.home.html; Paramecium http://paramecium.cgm.cnrs-gif.fr; Naegleria http://genome.jgi-psf.org/Naegr1/Naegr1.home.html; Trichomonas http://trichdb.org; and Trypanosoma https://www.sanger.ac.uk/resources/downloads/protozoa/trypanosoma-brucei.html.