Figure 3

TRPV4 plays a role in osteocyte mechanotransduction. (A,B) Percent of viable cells exhibiting flow-induced Ca²⁺ response as a function of peak amplitude (a multiple of the sample’s maximum baseline oscillation amplitude) (n = 15–21). (C , D) Timing of ciliary and cytosolic Ca²⁺ peaks of treated cells relative to untreated controls (untreated (n = 15), thapsigargin (n = 5 or 6)), Pkd2, Trpv4, and Piezo1 (n = 14–16). (E , F) Levels of normalized Cox-2 mRNA expression in scrambled siRNA and Trpv4 or Piezo1 siRNA-treated cells with and without 5 min of oscillatory flow exposure (n = 9–11). Error bars show ±SEM (*p < 0.05).