Fig. 5

Renal phenotype of the Bbs10 ^fl/fl ; Cadh16-Cre ^+/− mice. a PCR genotyping of heterozygous WT (Bbs10 ^+/+) (Bbs10 ^fl/+), floxed (Bbs10 ^fl/fl) mice non-Cre (Cre ^−/−) and heterozygous (Cre ^+/−) genotyping (bp base pairs). b 3D image of a kidney section immunostained for Cre (green) and nuclei counterstained with DAPI (blue). c 3D image of a kidney section immunostained for the primary cilia (acetylated α-tubulin in red), cytoskeleton (β-tubulin in green) and nuclei counterstained with DAPI (in blue). d Pictures of immunostained kidney sections for AQP2 (green) and β-tubulin (red) from Bbs10 ^fl/fl ; Cadh16Cre ^+/− mice. Nuclei were counterstained with DAPI (blue). Scale bars 20 µm. e T.E.M. analysis of epithelial cells in the proximal tubule from Bbs10 ^+/+ ; Cadh16Cre ^+/−and Bbs10 ^fl/fl ; Cadh16Cre ^+/− mice. BM basement membrane. f Microalbuminuria levels for of Bbs10 ^fl/fl ; Cadh16Cre ^+/− and control Bbs10 ^+/+ ; Cadh16Cre ^+/− littermates (n = 6–7 per genotype). g T.E.M. analysis of epithelial cells in the distal tubule from Bbs10 ^+/+ ; Cadh16Cre ^+/− and Bbs10 ^fl/fl ; Cadh16Cre ^+/− mice. h Creatinine clearance levels (µL/min) and urinary volume under fluid deprivation of Bbs10 ^fl/fl ; Cadh16Cre ^+/− and control Bbs10 ^+/+ ; Cadh16Cre ^+/− littermates (n = 7–9). Values are expressed as mean ± SEM. *p < 0.05.