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Fig. 5 | Cilia

Fig. 5

From: Current topics of functional links between primary cilia and cell cycle

Fig. 5

Effects of trichoplein depletion on cell cycle regulators. Proliferating RPE1 cells were transfected with siRNA for control or trichoplein (#1 and #2), and then cultured for 72 h. The cell extracts were subjected to immunoblotting analysis with p27Kip1, p53, p21Cip1, p16INK4a, trichoplein, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). As controls of immunoblotting with p27Kip1 or p53/p21Cip1, RPE1 cells were subjected to 72 h serum starvation or UV irradiation (10 J/m2, 24 h), respectively. These are original data for this review

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